Flow fluorometric method

摘要: This invention is related to a flow fluorometric device and method employing two-photon excitation and/or confocal optical set-up. The set-up of this optimal for counting small fluorescent biological particles. active focal volume diffraction-limited consequently much smaller than the channel. detection concept has been found very efficient rejection background signal. An objective lens with large numerical aperture focusing laser collecting fluorescence used restricts measurement which approximately corresponds femtoliter. significantly ordinary cytometry.