Molecular cloning of human cathepsin G: structural similarity to mast cell and cytotoxic T lymphocyte proteinases.
作者： Guy Salvesen , David Farley , Jon Shuman , Alan Przybyla , Christopher Reilly
DOI: 10.1021/BI00382A032
关键词:
摘要: Human cathepsin G is a serine proteinase with chymotrypsin-like specificity found in both polymorphonuclear leukocytes (neutrophils) and the U937 leukemic cell line. Utilizing RNA from latter, we have constructed cDNA library lambda gt11 isolated clone which apparently codes for complete amino acid sequence of this enzyme. Analysis reveals homology rat mast II (47%) but greater degree identity (56%) product activated mouse cytotoxic T lymphocytes. The close relationship between three proteins indicates similarities substrate biosynthesis predict involves removal two activation peptide during or just before packaging into their respective storage granules.
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William C. Merrick, Translation of exogenous mRNAs in reticulocyte lysates. Methods in Enzymology. ,vol. 101, pp. 606- 615 ,(1983) , 10.1016/0076-6879(83)01041-1
Richard G. Woodbury, Michael T. Everitt, Hans Neurath, [45] Mast cell proteases Methods in Enzymology. ,vol. 80, pp. 588- 609 ,(1981) , 10.1016/S0076-6879(81)80047-X
M M Gottesman, S Gal, The major excreted protein of transformed fibroblasts is an activable acid-protease. Journal of Biological Chemistry. ,vol. 261, pp. 1760- 1765 ,(1986) , 10.1016/S0021-9258(17)36004-0
E J Campbell, R M Senior, Cathepsin G in human mononuclear phagocytes: comparisons between monocytes and U937 monocyte-like cells. Journal of Immunology. ,vol. 132, pp. 2547- 2551 ,(1984)
Fernando C. Reinach, Donald A. Fischman, Recombinant DNA approach for defining the primary structure of monoclonal antibody epitopes. The analysis of a conformation-specific antibody to myosin light chain 2. Journal of Molecular Biology. ,vol. 181, pp. 411- 422 ,(1985) , 10.1016/0022-2836(85)90229-3
D A Portnoy, A H Erickson, J Kochan, J V Ravetch, J C Unkeless, Cloning and characterization of a mouse cysteine proteinase. Journal of Biological Chemistry. ,vol. 261, pp. 14697- 14703 ,(1986) , 10.1016/S0021-9258(18)66927-3
C F Reilly, D A Tewksbury, N M Schechter, J Travis, Rapid conversion of angiotensin I to angiotensin II by neutrophil and mast cell proteinases. Journal of Biological Chemistry. ,vol. 257, pp. 8619- 8622 ,(1982) , 10.1016/S0021-9258(18)34171-1
Robert M. Senior, Edward J. Campbell, Jill A. Landis, Fraya R. Cox, Charles Kuhn, Hillel S. Koren, Elastase of U-937 Monocytelike Cells: COMPARISONS WITH ELASTASES DERIVED FROM HUMAN MONOCYTES AND NEUTROPHILS AND MURINE MACROPHAGELIKE CELLS Journal of Clinical Investigation. ,vol. 69, pp. 384- 393 ,(1982) , 10.1172/JCI110462
N J Gay, J E Walker, Molecular cloning of a bovine cathepsin. Biochemical Journal. ,vol. 225, pp. 707- 712 ,(1985) , 10.1042/BJ2250707
P. J. Farrell, P. L. Deininger, A. Bankier, B. Barrell, Homologous upstream sequences near Epstein-Barr virus promoters Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 1565- 1569 ,(1983) , 10.1073/PNAS.80.6.1565