Energy Metabolism of Human Neutrophils during Phagocytosis

摘要: Detailed quantitative studies were performed on the generation and utilization of energy by resting phagocytosing human neutrophils. The ATP content was 1.9 fmol/cell, constant during rest, not influenced presence or absence glucose in medium. intracellular phosphocreatine less than 0.2 fmol/cell. In glucose, generated almost exclusively from lactate produced taken up surrounding amount could account for 85% cells, glycosyl store, glycogen, drawn upon. rate as calculated production 1.3 fmol/cell/min. During phagocytosis, there no measurable increase consumption production, fell rapidly to 0.8 This disappearance apparently irreversible since corresponding ADP AMP observed. It therefore appears that this phagocytosis-induced fall concentration represents all extra utilized neutrophils glucose. cell considerably smaller, 0.75 fmol/cell per min, glycolysis, which is sustained glycogenolysis. Under condition, however, phagocytosis induces significant enhancement glycogenolysis increased 60%, raising 1.2 min. Nonetheless, drops significantly 1.0 Neutrophils patients with chronic granulomatous disease have same glycolysis normal thus confirming defective respiration these cells does affect their metabolism.