Remote ischemic preconditioning with – but not without – metabolic support protects the neonatal porcine heart against ischemia-reperfusion injury

摘要: Abstract Background While remote ischemic preconditioning (rIPC) protects the mature heart against ischemia-reperfusion (IR) injury, effect on neonatal is not known. The relies almost solely carbohydrate metabolism, which modified by rIPC in heart. We hypothesized that combined with metabolic support glucose-insulin (GI) infusion improves cardiac function and reduces infarct size after IR injury piglets in-vivo. Methods results 32 newborn were randomized into 4 groups: control, GI, GI+rIPC rIPC. GI groups received continuously from 40min prior to ischemia. underwent four cycles of 5min limb Myocardial was induced occlusion left anterior descending artery followed 2h reperfusion. lactate concentrations assessed microdialysis samples analyzed mass spectrometry. Infarct measured using triphenyltetrazolium chloride staining. Systolic recovery (dP/dt max as % baseline) reperfusion 68.5±13.8% 53.7±11.2% ( p Conclusion rIPC+GI porcine alone has detrimental functional effects are abrogated simultaneous infusion.