Characterization of Differentially Expressed Genes following Brief Cardiac Ischemia

摘要: Abstract For the study of ischemia-related adaptations we employed DDRT-PCR on mRNA obtained from heart regions undergoing brief (10′) occlusions/reperfusions. We found and sequenced 52 differentially expressed clones that further characterized using Northern analysis with RNA a wide variety tissues. selected only (1) new sequence, (2) showed signal, (3) exhibited organ-specific (heart, muscle) or (4) situation-specific (ischemia, non-specific stress) expression. two heart-specific transcripts three stress-inducible genes. The known sequences an expression pattern typical for repair processes after ischemia-reperfusion (ubiquitin, ATPases). About 20% were truly expressed.