A simple procedure for the isolation of rat liver microsomes

摘要: Endoplasmic reticulum of rat liver undergoes extensive fragmentation upon homogenization [l] . The major component endoplasmic which accounted for lo-13% total was recognized as microsomes in early 194 1 [2] were reported to have variable size from 50300 nm [3], necessitating a high centrifugal force isolate them contrast other subcellular fractions like nuclei and mitochondria. standard procedure, thus requires sophisticated instrument an ultracentrifuge and, at the same time, is very time consuming. Taking advantage one characteristic properties skeletal muscle [4, 51 bind calcium ions, we report this communication relatively simple method isolation microsomes.