Investigation of the mechanism of the metabolic activation of chloramphenicol by rat liver microsomes: Identification of a new metabolite
作者： Lance R. Pohl , Sidney D. Nelson , Gopal Krishna
DOI: 10.1016/0006-2952(78)90383-0
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摘要: Abstract A new metabolite of chloramphenicol (CAP) has been isolated from incubation mixtures containing CAP and liver microsomes phenobarbital-pretreated rats. This compound identified as the oxamic acid derivative by reverse isotope dilution, high pressure liquid chromatography, isobutane chemical ionization mass spectroscopy. It also a product hydrolysis radiolabeled microsomal protein. These results support proposal that is metabolically activated to an oxamyl chloride reactive intermediate which either hydrolyzes or acylates
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