Étude de l'adsorption et de la pénétration du virus rabique: Interactions avec les cellules BHK21 et des membranes artificielles

摘要: Summary The binding of rabies virus and its entry into susceptible cells was studied using BHK21 a liposomal membrane model cellular constituents. When were incubated with radiolabelled or purified glycoprotein, viral material to cell surfaces observed. Saturation sites by unlabelled glycoprotein did not inhibit virus. This result suggested that the and/or mechanism molecules independant, depending on whether free in incubating solution anchored membrane. Binding enhanced virus-cell mixtures acidic conditions: amount radioactivity bound 10-fold higher at pH 5.4 than 7.2, but infectivity same. Furthermore, these two phenomenons required energy because lower amounts detected when 4°C (instead 20°C) sodium azide. Attempts saturate led types results: (1) inhibition labelled low concentrations. Rabies also liposomes prepared either lipids alone 0.01% Triton-X100 extracts. specific only 7.2 containing detergent extract. Under conditions, could be partially saturated before contact observation suggests bind detergent-soluble receptor. All results us propose attachment BHK21-cell surface requires receptor mainly involves fusion plasma