Method for electrochemically detecting nucleic acid-oligomer hybridisation events

摘要: The invention relates to a method for electrochemically detecting sequence-specific nucleic acid-oligomer hybridisation events. DNA/RNA/PNA oligomer single strands which are bound conductive surface at one end and linked redoxactive unit the other, free end, serve as matrix (probe). A proportion of strand oligonucleotides hybridised by treatment with oligonucleotide solution (target) being tested, result that electrical communication between unit, is initially non- or barely existent, increased. This enables event be detected using electrochemical methods such voltammetry, amperometry conductance measurement.