Long-Term Proliferation of Human Melanocytes Is Supported by the Physiologic Mitogens α-Melanotropin, Endothelin-1, and Basic Fibroblast Growth Factor

摘要: We hove successfully established normal neonatal and adult human melanocyte cultures in a growth medium containing the physiologic mitogens basic fibroblast factor (bFGF; 0.6 ng/ml), endothelin-1 (endo-1; 10 nM), α-melanocyte stimulating hormone (α-MSH; nM). The latter two factors replaced commonly used 12-O-tetradecanoylphorbol 13-acetate (TPA) bovine pituitary extract (BPE), respectively. Basic FGF alone maintained viability but did not induce proliferation of melanocytes. addition endo-1 to bFGF-containing resulted reduction tyrosinase activity without enhancement proliferation. However, α-MSH potentiated activity. concomitant endo-1, α-MSH, bFGF significantly increased entry melanocytes into S phase their Melanocytes under these conditions had same as those bFGF. signal transduction pathway induced by either or bFGF, includes activation mitogen-activated (MAP) kinase pathway. both more than an additive effect on MAP extracellular signal-regulated 2 (ERK2). This was further factors. In summary, we have devised for based use that substituted routinely artificial undefined resulting should be desirable clinical uses permissive expression vivo relevant responses regulatory