Identification of bloodmeals in wild caught blood fed Phlebotomus argentipes (Diptera: Psychodidae) using cytochrome b PCR and reverse line blotting in Bihar, India.

摘要: Identification of the source bloodmeals in vectors plays an important role epidemiological studies by determining host preferences wild sand flies natural habitat. The anthropophilic index is a crucial component human leishmaniasis. Bloodmeal analysis can identify reservoir hosts vector borne diseases. amplification mitochondrial cytochrome b gene, followed reverse line blot analysis, helps to bloodmeal ingested caught flies. In current study, blood fed were collected from three different villages Bihar, India, using Centers for Disease Control mini traps with incandescent light. Traps placed five sites villages. Whole genome DNA was extracted and amplified region, analysis. total, 442 analyzed out which 288 (65%) positive polymerase chain reaction. Humans, cattle, buffalo, goats major identified, chickens. some flies, multiple identified. mostly on humans, goats. this regard, it necessary also consider when addressing control India.