Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene

摘要: An analysis of the dietary content haematophagous insects can provide important information about transmission networks certain zoonoses. The present study evaluated potential polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) mitochondrial cytochrome B (cytb) gene to differentiate between vertebrate species that were identified as possible sources sandfly meals. complete cytb sequences 11 available in National Center for Biotechnology Information database digested with Aci I, Alu Hae III and Rsa I restriction enzymes silico using Restriction Mapper software. (358 bp) was amplified from tissue samples contents sandflies enzymes. Vertebrate presented a profile differed other species, exception Canis familiaris Cerdocyon thous. 358 bp 76 sandflies. Of these, 10 food predicted four: Homo sapiens (1), Bos taurus (1) Equus caballus (2). Thus, PCR-RFLP technique could be method identifying arthropods. However, some points must clarified regarding applicability method, such extent DNA degradation through intestinal digestion, multiple blood meals need greater knowledge intraspecific variations mtDNA.