Tracking the Feeding Patterns of Tsetse Flies (Glossina Genus) by Analysis of Bloodmeals Using Mitochondrial Cytochromes Genes

摘要: Tsetse flies are notoriously difficult to observe in nature, particularly when populations densities low. It is therefore them on their hosts nature; hence vertebrate species can very often only be determined indirectly by analysis of gut contents. This knowledge a critical component the information which control tactics developed. The objective this study was determine sources tsetse bloodmeals, investigate feeding preferences. We used mitochondrial cytochrome c oxidase 1 (COI) and b (cytb) gene sequences for identification fly blood meals, order provide foundation rational decisions guide trypanosomiasis, vectors. Glossina swynnertoni were sampled from Serengeti (Tanzania) G. pallidipes Kenya (Nguruman Busia), Uganda. Sequences query public databases, percentage identities obtained identify hosts. An initial assay showed that feeds single sources. Hosts identified fed collected ecosystem, included buffaloes (25/40), giraffes (8/40), warthogs (3/40), elephants (3/40) one spotted hyena. In Nguruman, where analyzed, (6/13) (5/13), while baboons accounted bloodmeal each. Only cattle detected caught Busia Out four tested Mbita Point, Suba District western Kenya, had cattle, other three Nile monitor lizard. These results demonstrate will form an integral part strategy trypanosomiasis Uganda, different approaches required Nguruman ecosystems, wildlife abound major food source.