Disentangling Vector-Borne Transmission Networks: A Universal DNA Barcoding Method to Identify Vertebrate Hosts from Arthropod Bloodmeals

摘要: Emerging infectious diseases represent a challenge for global economies and public health. About one fourth of the last pandemics have been originated by spread vector-borne pathogens. In this sense, advent modern molecular techniques has enhanced our capabilities to understand vector-host interactions disease ecology. However, host identification protocols poorly profited international DNA barcoding initiatives and/or focused exclusively on limited array vector species. Therefore, ascertaining potential afforded tools in other systems human veterinary importance would major advance tracking pathogen life cycles hosts. Here, we show applicability novel efficient method vertebrate host's contained midgut blood-feeding arthropods. To end, designed eukaryote-universal forward primer vertebrate-specific reverse selectively amplify 758 base pairs (bp) mitochondrial Cytochrome c Oxidase Subunit I (COI) gene. Our was validated using both extensive sequence surveys from domain Polymerase Chain Reaction (PCR) experiments carried out over specimens different Classes vertebrates (Mammalia, Aves, Reptilia Amphibia) invertebrate ectoparasites (Arachnida Insecta). The analysis mosquito, culicoid, phlebotomie, sucking bugs, tick bloodmeals revealed up 40 hosts, including 23 avian, 16 mammalian reptilian Importantly, inspection direct sequencing electropherograms also assisted resolving mixed bloodmeals. We therefore provide universal high-throughput diagnostic tool study ecology haematophagous invertebrates relation their Such information is crucial support management aimed at reducing epidemiologic risks arthropod pathogens, priority