Molecular detection and identification of Leishmania spp. in naturally infected Phlebotomus tobbi and Sergentomyia dentata in a focus of human and canine leishmaniasis in western Turkey.

摘要: Human visceral leishmaniasis (VL) is reported from 38 provinces of Turkey and dogs are accepted as main reservoir hosts. Kusadasi town, belonging to Aydin province located in western part Turkey, endemic for human canine caused by Leishmania infantum MON1 MON98. In this study, phlebotomine survey was conducted determine the vector sand fly species identify blood meal sources. August September 2012, 1027 specimens were caught using CDC light traps. Eight Phlebotomus two Sergentomyia with dominancy tobbi (61.34%) detected. A total 622 female flies (571 Phlebotomus; 51 Sergentomyia) checked infection direct dissection midgut. The half midgut content inoculated into NNN culture isolation parasite. species-specific ITS1 real time PCR, conventional PCR assays hsp70 genes subsequent sequencing performed extracted DNAs. region cytochrome b (cyt-b) gene vertebrates based used source flies. microscopical examinations, (0.32%) found naturally infected high number different stages promastigotes. No growth observed but DNA obtained both specimens. First positive specimen identified P. L. Second dentata, could not be on level. 16 blood-fed analysis eight, three one human, dog mouse, respectively. This first detection promastigotes examination S. dentata area Turkey. Our results indicate that, (i) principal (ii) sp. may an evidence natural cycle Sauro-leishmania agents area.