Environmental resistome risks of wastewaters and aquatic environments deciphered by shotgun metagenomic assembly
作者： Mutshiene Deogratias Ekwanzala , John Barr Dewar , Maggy Ndombo Benteke Momba
DOI: 10.1016/J.ECOENV.2020.110612
关键词:
摘要: Abstract In this paper, we deciphered the core resistome disseminating from hospital wastewater to aquatic environment by characterising resistome, plasmidome, mobilome and virulome using metagenomic analysis. This study also elucidated different environmental risks shotgun-metagenomic assembly. The results showed that clinically relevant taxa were found in assessed matrices (Salmonella spp., Acinetobacter spp, Escherichia-Shigella Pseudomonas Staphylococcus spp. Vibrio spp.). For 249 plasmidome sequences shared among all matrices. of 2424 mobile genetic elements was found. Regarding virulome, 148 virulence factors samples, content consistently across most abundant bacterial genera. Although influent considerable higher relative abundance (P = 0.008), significant risk scores against other matrices, with an average 46.34% (P = 0.001). These suggest wastewater, effluent sewage sludge should be subjected stringent mitigating measures minimise such dissemination.
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