Isolation and characterization of a collagenolytic enzyme from Bacillus licheniformis N22
作者： Somsit Asdornnithee , Kouichi Akiyama , Takeshi Sasaki , Renkichi Takata
DOI: 10.1016/0922-338X(94)90358-1
关键词:
摘要: A bacterium having collagenolytic activity was isolated from soil and identified as Bacillus licheniformis. The enzyme obtained in a medium containing gelatin purified to apparent homogeneity on the basis of SDS-polyacrylamide gel electrophoresis. had molecular weight approximately 29,000, hydrolyzed native, insoluble collagen. In addition, soluble collagen, gelatin, hemoglobin casein, but not synthetic oligopeptides, usually used substrates for collagenase assay.
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