Microfluidic label-free bioprocessing of human reticulocytes from erythroid culture

摘要: In vitro erythroid cultures from human hematopoietic stem cells produce immature red blood (RBCs) called reticulocytes, which are important for RBCs production, and widely used in scientific studies of malaria pathology, hematological diseases protein translation. However, reticulocyte contain expelled cell nuclei erythroblasts as undesirable by-products current purification methods such density gradient centrifugation fluorescence-activated sorting (FACS) not optimal integrated bioprocessing downstream therapeutic applications. Developments Dean flow fractionation (DFF) deterministic lateral displacement (DLD) microfluidic ideal alternatives due to label-free size sorting, throughput scalability low manufacturing cost. DFF reticulocytes whole culture showed a 2.4-fold increase recovery compared FACS albeit with lower purity; DLD comparable purity using an inverse-L pillar structure emphasize deformability reticulocytes. The viability functional assurance purified conserved supported the propagation parasites. Collectively, our study on isolation represents significant technical advancement towards developing generated viable RBCs, opening opportunities close-loop manufacturing, research purposes.