The Influence of the Proliferating Cell Nuclear Antigen-interacting Domain of p21CIP1 on DNA Synthesis Catalyzed by the Human and Saccharomyces cerevisiae Polymerase δ Holoenzymes

摘要: In eukaryotes, processive DNA synthesis catalyzed by polymerases delta and epsilon (pol epsilon) requires the proliferating cell nuclear antigen (PCNA). It has recently been shown that in humans (h), PCNA function, required for both replication nucleotide excision repair, can be inactivated p21(CIP1) due to a specific interaction between hPCNA carboxyl terminus of p21(CIP1). this report, we show Saccharomyces cerevisiae (S. cerevisiae) PCNA-dependent pol delta-catalyzed was inhibited less efficiently than human system intact protein unaffected carboxyl-terminal peptide (codons 139-160). This species-specific response p21(CIP1)-mediated inhibition results from marked difference ability h S. interact with As binding studies using surface plasmon resonance technique, binds full-length peptide-(139-160) stoichiometrically similar affinity (KD approximately 2.5 nM) while 10-fold does not peptide-(139-160).