Comparison between the retrograde axonal transport of nerve growth factor and tetanus toxin in motor, sensory and adrenergic neurons

摘要: In previous studies it has been shown that nerve growth factor (NGF) is taken up with high selectivity by adrenergic and sensory terminals transported retrogradely to the corresponding cell bodies a colchicine sensitive mechanism 10,11,23. The present study was designed investigate whether this retrograde transport of NGF depends on properties which are common all neurons or restricted those respond either during whole life cycle (adrenergic neurons) period embryonic development (sensory neurons). order in motor we injected [125I]NGF into musculus deltoideus measured side differences accumulation radioactivity spinal cord (C6-C8) from 4-48 h. At no time there preferential side. contrast, tetanus toxin accumulated preferentially an approximate rate 7.5 mm/h calculated. Astonishingly also neurons. identical could be blocked transection fibers local administration colchicine. After unilateral injection [125I]tetanus forepaw light microscopic autoradiographs revealed heavily labeled neuronal dorsal root ganglia ventrolateral It concluded membrane specific for neurons, whereas determined features all, at least peripheral, fact both examined supports hypothesis specificity uptake sites system non-specific.