The induction of mutants of bacteriophage T2 by 5-bromouracil

摘要: A kinetic study has been made of the effects 5-bromouracil deoxyriboside (BrUDR) on T2 development in a sulfanilamide-containing medium. As tested by u.v. light sensitivity intracellularly developing phage and time appearance first intracellular mature phages, it was found that sulfanilamide BrUDR did not change pattern development, but slowed down slightly. Sulfanilamide caused lysis inhibition r2 pahges this begin to be effective as mutagen until expected for commencement DNA synthesis, mutagenic action could exerted at any thereafter long continued synthesized. Counteraction free thymidine 15 min after infection halt production mutants. It is proposed mutations are induced both process introduction BrU into during replication containing BrU. Two mechanisms which might induce altered base sequences outlined.