Basic fibroblast growth factor-syndecan complex at cell surface or immobilized to matrix promotes cell growth.

摘要: Promotion of cell growth and differentiation by factors during early development organ formation are both temporally spatially very precise. Syndecan is a well characterized integral membrane proteoglycan that binds several extracellular matrix components via its heparan sulfate chains therefore suggested to participate in regulation. Syndecan-like molecules, as low affinity receptors for heparin-binding factors, have been recently also regulate factor activity. Heparin/heparan interaction required before, e.g. basic fibroblast (bFGF) can associate with high surface trigger signal transduction. In this paper we show syndecan, but not free chains, simultaneously bind bFGF molecules. Moreover, increased DNA synthesis 3T3 cells was observed when the were exposed beads coated fibronectin-syndecan-bFGF complex, indicating remains biologically active even immobilized syndecan. Finally, bound another type (epithelial), co-culture stimulated growth. Therefore, suggest syndecan-like molecules may determine sites action at cell-matrix cell-cell interfaces.