Chronic Treatment with a Clinically Relevant Dose of Methylphenidate Increases Glutamate Levels in Cerebrospinal Fluid and Impairs Glutamatergic Homeostasis in Prefrontal Cortex of Juvenile Rats.

摘要: The understanding of the consequences chronic treatment with methylphenidate is very important since this psychostimulant extensively prescribed to preschool age children, and little known about mechanisms underlying persistent changes in behavior neuronal function related use methylphenidate. In study, we initially investigate effect early on amino acids profile cerebrospinal fluid prefrontal cortex juvenile rats, as well glutamatergic homeostasis, Na+,K+-ATPase function, balance redox rats. Wistar rats at received intraperitoneal injections (2.0 mg/kg) or an equivalent volume 0.9 % saline solution (controls), once a day, from 15th 45th day age. Twenty-four hours after last injection, animals were decapitated obtained. Results showed that altered acid fluid, increasing levels glutamate. Glutamate uptake was decreased by administration, but GLAST GLT-1 not treatment. addition, astrocyte marker GFAP MPH. activity immunocontent catalytic subunits (α1, α2, α3) subjected treatment, α1 α2 gene expression α also observed. CAT increased SOD/CAT ratio sulfhydryl content rat cortex. Taken together, our results suggest induces excitotoxicity, least part, due inhibition glutamate probably caused disturbances and/or protein damage observed