Psychrophilic yeasts in glacial environments of Alpine glaciers

作者: Benedetta Turchetti , Pietro Buzzini , Marta Goretti , Eva Branda , Guglielmina Diolaiuti

DOI: 10.1111/J.1574-6941.2007.00409.X

关键词:

摘要: The presence of psychrophilic yeasts in supra- and subglacial sediments, ice meltwater collected from two glaciers the Italian Alps (Forni Sforzellina – Ortles-Cevedale group) was investigated. After incubation at 4 °C, sediments contained 1.3 × 103 to 9.6 103 CFU yeasts g−1. number yeast cells supraglacial c. 10–100-fold lower. A significant proportion isolated exhibited one or more extracellular enzymatic activities (starch-degrading, lipolytic, esterolytic, proteolytic pectinolytic activity) 4 °C. Selected isolates were able grow 2 °C under laboratory-simulated situ conditions. In all, 106 identified by MSP-PCR fingerprinting 26S rRNA gene sequencing D1/D2 region as belonging 10 species: Aureobasidium pullulans, Cryptococcus gilvescens (over 50% total), terricolus, Mrakia gelida, Naganishia globosa, Rhodotorula glacialis, psychrophenolica, bacarum, creatinivora laryngis. Four strains, all a new species, yet be described, also isolated.

参考文章(36)
Peter Raspor, Jure Zupan, Yeasts in Extreme Environments Springer, Berlin, Heidelberg. pp. 371- 417 ,(2006) , 10.1007/3-540-30985-3_15
D. Yarrow, Methods for the isolation, maintenance and identification of yeasts The Yeasts (Fourth Edition)#R##N#A Taxonomic Study. pp. 77- 100 ,(1998) , 10.1016/B978-044481312-1/50014-9
M. N. Poglazova, I. N. Mitskevich, S. S. Abyzov, M. V. Ivanov, [Microbiological characterization of the accreted ice of subglacial Lake Vostok, Antarctica]. Microbiology. ,vol. 70, pp. 838- 846 ,(2001) , 10.1023/A:1013148101626
Diego Libkind, Silvia Brizzio, Alejandra Ruffini, Mário Gadanho, Maria van Broock, José Paulo Sampaio, Molecular characterization of carotenogenic yeasts from aquatic environments in Patagonia, Argentina. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 84, pp. 313- 322 ,(2003) , 10.1023/A:1026058116545
Virginia De García, Silvia Brizzio, Diego Libkind, Pietro Buzzini, María Van Broock, Biodiversity of cold-adapted yeasts from glacial meltwater rivers in Patagonia, Argentina. FEMS Microbiology Ecology. ,vol. 59, pp. 331- 341 ,(2007) , 10.1111/J.1574-6941.2006.00239.X
N. Gunde-Cimerman, S. Sonjak, P. Zalar, J.C. Frisvad, B. Diderichsen, A. Plemenitaš, Extremophilic fungi in arctic ice: a relationship between adaptation to low temperature and water activity Physics and Chemistry of The Earth. ,vol. 28, pp. 1273- 1278 ,(2003) , 10.1016/J.PCE.2003.08.056
Lorena Butinar, Isabel Spencer-Martins, Nina Gunde-Cimerman, Yeasts in high Arctic glaciers: the discovery of a new habitat for eukaryotic microorganisms Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 91, pp. 277- 289 ,(2007) , 10.1007/S10482-006-9117-3
Brent C Christner, Ellen Mosley-Thompson, Lonnie G Thompson, Victor Zagorodnov, Kathleen Sandman, John N Reeve, Recovery and Identification of Viable Bacteria Immured in Glacial Ice Icarus. ,vol. 144, pp. 479- 485 ,(2000) , 10.1006/ICAR.1999.6288
Warwick F. Vincent, Microbial ecosystems of Antarctica ,(1988)
K. Pavlova, D. Grigorova, T. Hristozova, A. Angelov, Yeast strains from Livingston Island, Antarctica. Folia Microbiologica. ,vol. 46, pp. 397- 401 ,(2001) , 10.1007/BF02814428