The Intranuclear Binding of Testosterone and 5α-Androstan-17β-ol-3-one by Rat Prostate

摘要: Abstract The intranuclear binding of radioactive hormone in prostate after the intravenous administration testosterone-1,2-3H to rats has been studied. Nuclei obtained by sucrose density gradient centrifugation were extracted with buffer containing 0.6 m NaCl, and soluble radioactivity was separated into bound free fractions gel filtration on Sephadex G-25 or G-200. As early as 15 min testosterone administration, recovered form predominantly dihydrotestosterone. By nuclear extracts G-200 use digestive enzymes, it shown that dihydrotestosterone an acidic protein. Finally, several characteristics this phenomenon defined; stable freezing for long 8 days, short term incubation at 20° but not 37°, partially repeated Sephadex.