Biliary lipid output in the early stage of acute liver failure induced by 90% hepatectomy in the rat.

摘要: Background Differences in biliary lipid output were compared rats after 70% or 90% hepatectomy (Hx) to evaluate a possible index of the early stage acute liver failure. Methods Male Sprague-Dawley (SD) weighing 300 350 g randomly divided into two groups for Hx Hx, and animals sacrificed at 0, 6, 24, 48 h Hx. Before sacrifice, polyethylene tube was cannulated bile duct collected 1 h. Outputs total acids, phospholipid, cholesterol serum determined. Biliary cholesterol, acid concentrations, component levels determined using gas liquid chromatography. Hepatic microsomal 7α-hydroxylase sterol 12α-hydroxylase activities also high performance Results The 3-day survival rate 50%. In group, concentration each point significantly higher than it group. flow outputs acids lower 6 Among species, cholic chenodeoxycholic less decreased Conclusions Our results suggest that determinations are supposed be useful detection hepatic failure extensive hepatectomy.