Regulation of α-fetoprotein and transferrin synthesis and secretion in mouse hepatoma cells

摘要: Abstract Significant differences in the glucocorticoid- and cyclic nucleotide-mediated regulation of secretory glycoproteins, α-fetoprotein transferrin, have been observed to develop a mouse hepatoma cell line, Hepa-2, after many passages culture. Treatment low-passage cells with hydrocortisone (10 −6 m ), N 6 ,O 2 -dibutyryl AMP −3 or 8-bromo-cyclic ) results 1.5-, 2- 4-, 5.5- 6-fold increases, respectively, rates synthesis secretion α-fetoprotein. As expected proteins, ratio is 1 remains unaltered when treatment hydrocoritsone, AMP, causes stimulation secretion. Similar studies showing that albumin transferrin are also balanced these published indicate coupled cells. In high-passage Hepa-2 cells, however, we shown relative rate higher than its 4. Similarly, 3.6, whereas it for albumin. When treated there greater increase both resulting reduction from 4 1.63 3.6 2.3 transferrin. This effect on specific nucleotides occurs only Hydrocortisone an However, increases 3.96 control 5.5 Our show, therefore, this because slightly which not reflected exerts 6.2. We propose during continued subculturing variant selected serum glycoprotein uncoupled. Furthermore, glycoproteins since by remained unaltered.