B-cell epitopes of the envelope glycoprotein of caprine arthritis-encephalitis virus and antibody response in infected goats.

摘要: Goats infected with caprine arthritis–encephalitis virus (CAEV) develop high titres of antibodies to Env. Not only is no consistent neutralizing response found but anti-Env have even been associated disease in goats. To identify the continuous antigenic determinants involved this atypical response, we mapped CAEV-CO Env by screening an epitope expression library goat sera. In addition four previously described epitopes, seven novel sites were identified, which five located on surface (SU) and two transmembrane (TM) subunits The SU antibody-binding domains variable regions C-terminal part molecule (SU3 SU5) showed strongest reactivity induced a rapid seroconversion six experimentally However, these immunodominant epitopes did not appear be any activity. pattern serum naturally goats was restricted, suggesting type-specific reaction. Interestingly, peptides representing SU5 sequences derived from CAEV field isolates varied geographical and/or breeding origin animals. This suggests that corresponding may well useful serotyping isolates. Furthermore, identification will permit us functionally dissect antibody address role either protection or pathogenesis infection.