Evaluation of PCR-based methods for the diagnosis of tuberculosis by identification of mycobacterial DNA in urine samples.

摘要: Setting The Chest Clinic and the JICA (Japan International Cooperation Agency) Molecular Laboratories, University Teaching Hospital, Lusaka, Zambia, Department of Internal Medicine, Institute Tropical Nagasaki University, Nagasaki, Japan. Objective To evaluate polymerase chain reaction (PCR) as a laboratory test for rapid diagnosis pulmonary tuberculosis in African situation by identifying mycobacterial DNA urine samples using two commonly described molecular methods. Design Prospective collection analysis from adult Zambian patients with culture-confirmed healthy controls. Methods Urine was obtained 63 active 'healthy' control no tuberculosis. isolated sediment subjected to analyses well-described PCR-based methods, 'the Sechi method' Githui method', identification Mycobacterium DNA. sensitivity specificity tests were determined. Results method 55.6% (35/63) 98.4% (62/63), respectively. 28.6% (18/63) Of patients, 50 (79%) HIV sero-positive frequency positive PCR urines greater HIV-positive than HIV-negative (32/50 = 64% vs. 3/13 23%; P 0.05). Conclusions Neither nor sensitive enough be recommended routine use clinical practice. assays detection M. will require further refinement before they can practice Africa. presence also requires study.