Genomic Insights Into Energy Metabolism of Carboxydocella thermautotrophica Coupling Hydrogenogenic CO Oxidation With the Reduction of Fe(III) Minerals.

摘要: The genus Carboxydocella forms a deeply branching family in the class Clostridia and is currently represented by three physiologically diverse species of thermophilic prokaryotes. type strain species, thermautotrophica 41T, an obligate chemolithoautotroph growing exclusively hydrogenogenic CO oxidation. Another strain, isolated from hot spring at Uzon caldera, Kamchatka course this work, capable coupling carboxydotrophy dissimilatory reduction Fe(III) oxic phyllosilicate minerals. processes appeared to be interdependent strain. genomes both isolates were sequenced, assembled into single chromosome sequences (for 41T plasmid sequence was also assembled) analyzed. Genome analysis revealed that each two strains possessed six genes encoding Ni,Fe-containing dehydrogenases (maximum reported complete prokaryotic genomes), indicating crucial role carbon monoxide C. metabolism. Both set 30 multiheme c-type cytochromes, but only newly Fe-reducing 019 had one extra gene 17-heme cytochrome, which proposed represent novel determinant iron Mossbauer studies induced reductive transformation abundant ferric/ferrous-mica mineral glauconite siderite during carboxydotrophic growth. Reconstruction energy metabolism first comprehensive genome representative deep phylogenetic branch Incertae Sedis, V. Our data provide insights with emphasis on its ecological implications.