Histone H1 Subtypes Differentially Modulate Chromatin Condensation without Preventing ATP-Dependent Remodeling by SWI/SNF or NURF
作者： Jaime Clausell , Nicole Happel , Tracy K. Hale , Detlef Doenecke , Miguel Beato
DOI: 10.1371/JOURNAL.PONE.0007243
关键词:
摘要: Although ubiquitously present in chromatin, the function of linker histone subtypes is partly unknown and contradictory studies on their properties have been published. To explore whether various H1 a differential role organization dynamics chromatin we incorporated all somatic human into minichromosomes compared influence nucleosome spacing, compaction ATP-dependent remodeling. exhibit different affinities for abilities to promote condensation, as studied with Atomic Force Microscope. According this criterion, can be classified weak condensers (H1.1 H1.2), intermediate (H1.3) strong (H1.0, H1.4, H1.5 H1x). The variable C-terminal domain required spacing by H1.4 likely responsible condensation subtypes, shown using chimeras between H1.2. In contrast previous reports isolated nucleosomes or linear nucleosomal arrays, histones at ratio one per do not preclude remodeling yeast SWI/SNF Drosophila NURF. We hypothesize that are organizers rather than general repressors.
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