SEPARATION OF THE TOXIN OF BACILLUS CEREUS INTO TWO COMPONENTS AND NONIDENTITY OF THE TOXIN WITH PHOSPHOLIPASE

摘要: Molnar, Dorothy M. (U.S. Army Chemical Corps, Fort Detrick, Frederick, Md.). Separation of the toxin Bacillus cereus into two components and nonidentity with phospholipase. J. Bacteriol. 84:147-153. 1962-Bacillus produced in a Casamino acids medium without added serum or other protein. The was separated by adsorption on columns calcium phosphate gel followed elution buffer (pH 7.5). component eluted first has been called factor I later II. When tested alone each relatively nontoxic, but when combined they formed toxic mixture as evidenced skin reactions guinea pigs tests for lethal effects mice. phospholipase activity, determined egg-yolk reaction, found fraction containing I. However, using alumina-C(gamma) activity This suggests that is not same chemical entity either following are further evidence non-identity phospholipase: (i) differential precipitation activities (NH(4))(2)SO(4); (ii) neutralization various antisera; (iii) inhibition ethanol.