Force Nanoscopy of Hydrophobic Interactions in the Fungal Pathogen Candida glabrata

摘要: Candida glabrata is an opportunistic human fungal pathogen which binds to surfaces mainly through the Epa family of cell adhesion proteins. While some proteins mediate specific lectin-like interactions with epithelial cells, others promote and biofilm formation on plastic via nonspecific that are not yet elucidated. We report measurement hydrophobic forces engaged in Epa6-mediated by means atomic force microscopy (AFM). Using single-cell spectroscopy, we found C. wild-type (WT) cells attach strongly adhesive macromolecular bonds, while mutant impaired Epa6 expression weakly adhesive. Nanoscale mapping yeast using AFM tips functionalized groups shows massively exposed WT conveys strong properties surface. Our results demonstrate mediates interactions, thereby providing a molecular basis for ability this adhesin drive abiotic surfaces.