Real-Time Observation of Antimicrobial Polycation Effects on Escherichia coli: Adapting the Carpet Model for Membrane Disruption to Quaternary Copolyoxetanes

摘要: Real-time atomic force microscopy (AFM) was used for analyzing effects of the antimicrobial polycation copolyoxetane P[(C12)-(ME2Ox)-50/50], C12–50 on membrane a model bacterium, Escherichia coli (ATCC# 35218). AFM imaging showed cell changes with increasing concentration and time including nanopore formation bulges associated outer bacterial disruption. A macroscale bactericidal study 4 log kill at 15 μg/mL conditions paralleling (1 h, 1x PBS, physiological pH, 25 °C). The dramatic from control image to 1 h after introducing are therefore reasonably attributed death. At highest (60 μg/mL) further disruption results in leakage cytoplasm driven by detergent-like action. sequence processes initial synthetic follows carpet posited peptides (AMPs). However, nanoscale...