High frequency of IL-4-producing CD4+ allergen-specific T lymphocytes in atopic dermatitis lesional skin.

摘要: In atopic dermatitis (AD) hypersensitivity reactions to allergens are commonly observed and assumed make a major contribution in the pathomechanism of disease. It may be expected that allergen-reactive Th cells play central role these reactions. present study occurrence function allergen-specific T lymphocytes dermal inflammatory lesions were studied. To this aim panels randomly cloned CD4+ from lesional skin biopsies two housedust mite Dermatophagoides pteronyssinus (Dp)-allergic AD patients screened for reactivity with Dp allergens. The results compared similar tests T-lymphocyte clones (TLC) peripheral blood patients. TLC generated (S-TLC), considerable number appeared Dp-specific, 47% (n = 17) 10% 29), respectively. blood, percentages Dp-specific only 0% 22) 3% 34), suggesting accumulation or expansion skin. was studied by assaying secretion IL-4 IFN-gamma, which have been shown produced aberrant ratios (Wierenga et al: J Immunol 144:4651, 1990). All S-TLC combination no low levels whereas many non-Dp-specific blood-derived (B-TLC) produce high IFN-gamma without significant amounts IL-4. A functional consequence cytokine profiles demonstrated finding producing substantial enhanced expression low-affinity Fc receptor IgE (CD23) on antigen-presenting greater extent than did IFN-gamma-producing TLC.