Entamoeba histolytica: analysis of the trophozoite proteome by two-dimensional polyacrylamide gel electrophoresis.
作者： David Leitsch , Christian Radauer , Katharina Paschinger , Iain B.H. Wilson , Heimo Breiteneder
DOI: 10.1016/J.EXPPARA.2005.02.016
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摘要: Abstract The Entamoeba histolytica genome project carried out at TIGR and the Sanger Institute has produced a near-complete set of deduced open reading frame sequences. These data provide strong support for identification signals from proteomic analyses such as two-dimensional electrophoresis by protein sequencing and/or mass spectrometric methods. To carry an initial investigation E. proteome, appropriate sample preparation silver staining protocols were adapted. After extracts HM-1:IMSS trophozoites, solubilized proteins separated in first dimension IPG (immobilized pH gradient) strips depending on their pI subsequently second according to molecular weight SDS–polyacrylamide gel electrophoresis. Of more than 1500 spots visualized, several landmark isolated gels identified either tryptic cleavage subsequent MALDI-TOF spectrometry or sequencing.
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