Major role for cysteine proteases during the early phase of Acanthamoeba castellanii encystment.

摘要: Acanthamoeba castellanii is a facultative pathogen that has two-stage life cycle comprising the vegetatively growing trophozoite stage and dormant cyst stage. Cysts are formed when cell encounters unfavorable conditions, such as environmental stress or food deprivation. Due to their rigid double-layered wall, cysts highly resistant antiamoebic drugs. This problematic can survive initially successful chemotherapeutic treatment cause relapse of disease. We studied encystment process by using two-dimensional gel electrophoresis (2DE) found most changes in protein content occur early process. Truncated actin isoforms were abound encysting cell, levels translation elongation factor 2 (EF2) sharply decreased, indicating rate synthesis must be low at this In advanced encystment, however, EF2 proteome partly restored. The protease inhibitors PMSF (phenylmethylsulfonyl fluoride) E64d [(2S,3S)-trans-epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester] inhibited onset whereas inhibitor cycloheximide was ineffective. Changes profile, similar those cells, could observed with homogenates incubated room temperature for several hours. Interestingly, these significantly cysteine but not against other proteases. Taken together, we conclude A. bipartite nature consisting an initial phase autolysis degradation restoration accompanied expression encystment-specific genes.