Changes in profiles of major proteins in encysting acanthamoeba castellanii

摘要: The life cycle of Acanthamoeba is comprised two distinct stages, trophozoite and cyst. During periods stress, trophozoites undergo cellular differentiation into In order to understand the differentiation, we followed changes in profiles major proteins by 2D‐PAGE ubiqui‐tinated immunoblotting with anti‐ubiquitin (Ub) monoclonal antibody (mAb) as a probe. We observed 51 present were lost encystment. found that 43 within 24 h, 8 96 h Among them, 17 stained anti‐Ub mAb. cysts, 16 including 2 mAb‐reactive newly synthesized. Four detected h‐cyst disappeared h‐cyst, one protein was synthesized 24–96 168 11 appeared upon encystment all cyst stages. identified specific 33 kDa subtilis in‐like serine proteinase N‐terminal...