Optimal development of Chlamydophila psittaci in L929 fibroblast and BGM epithelial cells requires the participation of microfilaments and microtubule-motor proteins.

摘要: Abstract The cytoskeleton is involved in several cellular activities, including internalization and transport of foreign particles. Although particular functions to each component have been described, interactions between those components seem occur. involvement the different host cell cytoskeletal uptake development ofChlamydophila psittaci incompletely understood. In this study, participation microfilament network along with kinesin dynein microtubule motor proteins further Chlamydophila were investigated L929 fibroblast BGM epithelial cells. Cytochalasin D disruption actin filaments, blockage through introduction monoclonal antibodies into cells carried out, either single or combined, at moments around bacterial inoculation, infectivity determined 24 h post- inoculation by direct immunofluorescence. Our results show that, although Ipsittaci can make use both microfilament-dependent independent entry pathways types, mainly concerned processes mediated microfilaments while mechanisms that require ones predominantly involved. Evidence mutual tubulin networks are required for optimal growth also presented.