Compositional analysis of glycosaminoglycans by electrospray mass spectrometry.

摘要: The purpose of this work is to analyze glycosaminoglycans (GAGs) directly from complex mixtures without the need purify individual components. Novel conditions for negative ion electrospray MS chondroitin sulfate (CS) oligosaccharides are described in which sodium adduction and fragmentation avoided. Differentiation between positional sulfation isomers demonstrated CS disaccharides, a selected reaction monitoring scheme used quantify disaccharides liberated decorin biglycan. A size exclusion chromatography LC/MS method shown be effective compositional analysis longer oligosaccharides. SEC step serves simplify composition GAGs entering mass spectrometer at any time, thus allowing masses constituent molecules extracted. Mass spectrometric detection produces far more information than conventional UV or fluorescent detectors allows monosaccharide components determined.