Effector and regulatory dendritic cells display distinct patterns of miRNA expression.

摘要: Introduction MicroRNAs (miRNAs) contribute to the regulation of dendritic cell (DC) polarization, thereby influencing balance adaptive immune responses. Herein, we studied expression miRNAs in polarized DCs and analyzed whether these could be associated with allergic rhinitis allergen immunotherapy (AIT) outcome. Method Using specific culture conditions, differentiated immature human monocyte-derived into DC1, DC2, DCreg subsets (supporting differentiation TH1, TH2 or regulatory T cells, respectively). Profiling miRNA was performed DC subpopulations using microarrays. Levels for were then evaluated a cohort 58 patients 25 non-allergic controls, as well samples from 30 subjects treated sublingual grass pollen tablets placebo four months. Results We successfully identified 16 differentially regulated between DCs, cells. In rhinoconjunctivitis patients, two those (miR-132 miR-155), down-regulated compared individuals. However, levels not significantly modified following months immunotherapy. Conclusions Studying clinical without rhinoconjunctivitis, demonstrated that linked effector (i.e., DC1 and/or DC2 cells), reduced blood rhinoconjunctivitis. Nevertheless, did represent relevant biomarkers predict follow-up AIT efficacy.