The anti-oxidant effects of melatonin derivatives on human gingival fibroblasts.
作者： Chawapon Phiphatwatcharaded , Ploenthip Puthongking , Ponlatham Chaiyarit , Nutjaree Pratheepawanit Johns , Sumon Sakolchai
DOI: 10.1016/J.ARCHORALBIO.2017.02.022
关键词:
摘要: Abstract Objectives Aim of this in vitro study was to evaluate the anti-oxidant activity indole ring modified melatonin derivatives as compared with primary human gingival fibroblast (HGF) cells. Methods Anti-oxidant (MLT), acetyl-melatonin (AMLT) and benzoyl-melatonin (BMLT) evaluated by5 standard methods follows: 2, 2-diphenyl-1-picrylhydrazyl (DPPH); ferric ion reducing antioxidant power (FRAP); superoxide anion scavenging; nitric oxide (NO) thiobarbituric acid reactive substances (TBARs).Evaluation cellular (CAA) protectivity against H 2 O induced damage performed via MTT assay HGF Results According assays, AMLT BMLT were slightly less than MLT FRAP scavenging assays. In NO TBARs more potent MLT, whereas DPPH assays demonstrated that others. had protective activities cells MLT. Conclusions different depending upon These findings imply N- substitution may help improve hydrogen atom transfer free radicals but electron property is decreased. effects (AMLT BMLT) on fibroblasts potential use these molecules alternative therapeutics for chronic inflammatory oral diseases.
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