MicroRNA Stability in FFPE Tissue Samples: Dependence on GC Content

摘要: MicroRNAs (miRNAs) are small non-coding RNAs responsible for fine-tuning of gene expression at post-transcriptional level. The alterations in miRNA levels profoundly affect human health and often lead to the development severe diseases. Currently, high throughput analyses, such as microarray deep sequencing, performed order identify biomarkers, using archival patient tissue samples. MiRNAs more robust than longer RNAs, resistant extreme temperatures, pH, formalin-fixed paraffin-embedding (FFPE) process. Here, we have compared stability miRNAs FFPE cardiac tissues next-generation sequencing. mode read length samples was 11 nucleotides (nt), while that matched frozen 22 nt. Although counts were increased 1.7-fold samples, with those average mapping rate decreased from 32.0% 9.4%. These results indicate that, addition fragmentation some extent degraded well. profiles total two groups highly correlated (0.88