Simultaneous determination of cysteine sulfinic acid and hypotaurine in rat tissues by column-switching high-performance liquid chromatography with electrochemical detection.
作者： Tadayasu Togawa , Atsushi Ohsawa , Koji Kawanabe , Shinzo Tanabe
DOI: 10.1016/S0378-4347(97)00449-0
关键词:
摘要: Cysteine sulfinic acid (CSA) and hypotaurine (HT) were determined by electrochemical detection with a glassy carbon electrode at 0.95 V vs. Ag/AgCl. The separation of CSA HT was accomplished coupled-column liquid chromatography, consisting an anion-exchange column cation-exchange column. For the determination in rat tissues, column-switching system introduced to remove interferences from late-eluting endogenous substances. limits 0.05 μM for both acids. average precisions (C.V.) over concentration range 5 4.3% 4.2% HT.
elsevier.com
sci-hub.se 参考文章(10)
Noriyoshi Masuoka, Kazushi Ishino, Kenzaburoh Yao, Reiko Akagi, Toshihiko Ubuka, Preparation of a volatile derivative of taurine and application to gas chromatographic determination of urinary taurine. Acta Medica Okayama. ,vol. 43, pp. 253- ,(1989) , 10.18926/AMO/30881
Ryan J. Huxtable, Biochemistry of Sulfur ,(1986)
Owen W. Griffith, Mammalian sulfur amino acid metabolism: An overview Methods in Enzymology. ,vol. 143, pp. 366- 376 ,(1987) , 10.1016/0076-6879(87)43065-6
Lawrence L. Hirschberger, James de la Rosa, Martha H. Stipanuk, Determination of cysteinesulfinate, hypotaurine and taurine in physiological samples by reversed-phase high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 343, pp. 303- 313 ,(1985) , 10.1016/S0378-4347(00)84599-5
D. R. Curtis, J. C. Watkins, The excitation and depression of spinal neurones by structurally related amino acids. Journal of Neurochemistry. ,vol. 6, pp. 117- 141 ,(1960) , 10.1111/J.1471-4159.1960.TB13458.X
Noriyoshi Masuoka, Kenzaburoh Yao, Masahiro Kinuta, Jun Ohta, Masahiro Wakimoto, Toshihiko Ubuka, High-performance liquid chromatographic determination of taurine and hypotaurine using 3,5-dinitrobenzoyl chloride as derivatizing reagent. Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 660, pp. 31- 35 ,(1994) , 10.1016/0378-4347(94)00269-X
Shuji Ida, Kinya Kuriyama, Simultaneous determination of cysteine sulfinic acid and cysteic acid in rat brain by high-performance liquid chromatography. Analytical Biochemistry. ,vol. 130, pp. 95- 101 ,(1983) , 10.1016/0003-2697(83)90654-1
Hiroyuki Kataoka, Shigeo Yamamoto, Masami Makita, Quantitative gas—liquid chromatography of taurine Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 306, pp. 61- 68 ,(1984) , 10.1016/S0378-4347(00)80869-5
H. SHIMIZU, Y. YAMAMURA, EFFECTS OF DIAMINOPROPIONATE, DEOXYADENOSINE, AND THEOPHYLLINE ON STIMULATED FORMATION OF CYCLIC AMP AND GMP BY DEPOLARIZING AGENTS IN SLICES OF GUINEA-PIG CEREBRAL CORTEX Journal of Neurochemistry. ,vol. 28, pp. 383- 388 ,(1977) , 10.1111/J.1471-4159.1977.TB07758.X
C E Wright, H H Tallan, Y Y Lin, Taurine: Biological Update Annual Review of Biochemistry. ,vol. 55, pp. 427- 453 ,(1986) , 10.1146/ANNUREV.BI.55.070186.002235