High-performance liquid chromatographic determination of taurine and hypotaurine using 3,5-dinitrobenzoyl chloride as derivatizing reagent.
作者： Noriyoshi Masuoka , Kenzaburoh Yao , Masahiro Kinuta , Jun Ohta , Masahiro Wakimoto
DOI: 10.1016/0378-4347(94)00269-X
关键词:
摘要: Abstract A method for the determination of taurine and hypotaurine in biological samples involving preparation their 3,5-dinitrobenzoyl derivatives followed by HPLC was established. Taurine aqueous media were reacted with chloride presence triethylamine to prepare derivatives. These separated on a C18 reversed-phase column detected recording absorbance at 254 nm. Derivatives obtained yields 91.4 ± 3.3 85.6 2.6%, respectively. The calibration graphs linear between 2.5 500 μM correlation coefficients 0.999. applied human rat urine blood liver heart.
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