A direct determination of taurine in perchloric acid-deproteinized biological samples

摘要: Abstract A high-performance liquid chromatographic method was developed to estimate the amount of taurine in tissue extracts and body fluids. Shodex Ionpak C-811, an adsorption-distribution type column, chosen combined with 3 m perchloric acid as mobile phase. The eluted derivatized o-phthalaldehyde-2-mercaptoethanol reagent measured at absorbance 350 nm instead fluorescent intensity. Using this method, it possible isolate from other coexisting substances or structural analogs such hypotaurine, cysteamine, cysteinesulfinic acid. For rapid routine assays many biological samples, is advisable that be pretreated cation-exchange resin exclude interfering including O-phosphoserine. linear relationship between concentration achieved over range 0.1–10.0 nmol recoveries obtained were 98–101% by internal standard method. specificity, reproducibility, sensitivity for good enough used applications. Examples levels various organs rat, determined new are also presented.