Neural stem cell-based cell carriers enhance therapeutic efficacy of an oncolytic adenovirus in an orthotopic mouse model of human glioblastoma.

摘要: The potential utility of oncolytic adenoviruses as anticancer agents is significantly hampered by the inability currently available viral vectors to effectively target micrometastatic tumor burden. Neural stem cells (NSCs) have ability function cell carriers for targeted delivery an adenovirus because their inherent tumor-tropic migratory ability. We previously reported that in vivo CRAd-S-pk7, a glioma-restricted adenovirus, can enhance survival animals with experimental glioma. In this study, we show intratumoral NSCs loaded CRAD-S-pk7 orthotopic xenograft model human glioma able not only inhibit growth but more importantly increase median ~50% versus treated CRAd-S-pk7 alone (P = 0.0007). also report virus infection upregulates different chemoattractant receptors and enhances capacity both vitro vivo. Our data further suggest NSC-based improve clinical efficacy antiglioma virotherapy protecting therapeutic from host immune system, amplifying payload selectively at sites.