Reversibility of d-penicillamine induced collagen alterations in rat skin and granulation tissue

摘要: Abstract Granulation tissue was produced in rats by subcutaneous implantation of Visella ® sponges. d -penicillamine ( -pen) 100 or 500 mg/kg administered daily for 42 days gastric tubing. Pairfed, placebo treated animals were included as controls. Half the groups kept additionally 28 without medication. The inhibitory effect -pen on cross-link formation newly synthesized collagen readily reversible. By contrast, deficiency lasting beyond observation period observed higher polymeric variants released dilute acid, heat exposure limited pepsin proteolysis estimated solubility, α/β chain ratio and/or aldehyde content. SDS-polyacrylamide gel electrophoresis gels containing 3.6 M urea it shown that purified dermal acid soluble from consisted a mixture type I and III collagen, whereas only detected band pattern identical reduced unreduced samples. Four weeks after discontinuance had disappeared extract. Moreover, to digest both granulation skin showed persistent rise with -pen. These observations indicate destabilized particular interference its disulfide linkages. amount remained unaffected throughout experiment, content decreased at dose level. regeneration not completed end period. Modulation molecular stability granuloma collagens may be relevance antirheumatoid -pen, but sustained normal tissues imply long standing impairment their supportive capacity.