Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective

摘要: Summary Recent developments in industrial biotechnology have resulted the exploitation ofnew and undiscovered microorganisms devising of improved methods for en-zyme production, which led to increased yields enzyme, thus making a viableindustrial process feasible. This review tracks field acidic andneutral protease production with regard producers, andtheir improvement, product its applications. Key words : neutral protease, fermentation, genetic engineering, purifica-tion, enzyme assay Introduction Proteases are one industrially most importantenzymes. These proteolytic (protein digesting) biocata-lysts been use many centuries, at first thedairy industry as milk-clotting agents (rennet) themanufacture cheese. enzymes that cata-lyse hydrolytic reactions protein molecules aredegraded peptides amino acids. constitutea very large complex group enzymes, dif-fer properties such substrate specificity, active siteand catalytic mechanism, pH temperature optima andstability profile. The specificity isgoverned by nature acid other func-tional groups (aromatic or aliphatic sulphur-contain-ing) close bond being hydrolysed. arepresent all living beings play an important rolein normal abnormal physiological conditions, cata-lysing various metabolic (