Usefulness of nutritional screening for the identification of Xanthomonas campesths DNA homology groups and pathovars

摘要: A nutritional screen of 143 carbon sources was done on 88 strains xanthomonads from 39 different Xanthomonas campestris pathovars, X. albilineans, X.fragariae, and ‘X. gardneri’. Six compounds, cellobiose, fructose, fumarate, glucose, L-malate succinate supported growth all except whereas 92 substrates were not utilized by any strain. Substrate utilization patterns appeared sufficiently uniform among the various genomic groups within to allow their differentiation. The most easily distinguished pathovars cam. pv. oryzicola secalis 4 3, respectively, because they used few substrates. Genomic group 1 difficult distinguish differed substantially that comprise group. useful for distinguishing groups. For example, pelargonii 5 differentiated carotae, taraxaci, gardneri’ aconitate but D-tartrate. Similarly, use D-tartrate celebensis juglandis 6. Sorbitol only plantaginis 2 arabitol a substrate identifying pisi eucalypti. Most confirmed with Biolog tests there exceptions as found glycerol D-arabitol. test also revealed some differences in detected standard It is concluded screening has promise genus Xanthomonas.